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. Author manuscript; available in PMC: 2017 Jul 13.
Published in final edited form as: Cell Host Microbe. 2016 Jul 13;20(1):72–82. doi: 10.1016/j.chom.2016.06.006

Figure 5. TgIST associates with members of the Mi2/NuRD complex independently of STAT1 and IFN-γ activation.

Figure 5

(A) Western blot analysis of TgIST-Ty IP from Toxoplasma type I (RH) infected U3A (STAT1 null) or U3A-STAT1 (STAT1+) cells that were either left naive (−) or activated (+) with IFN-γ (100 U/ml) for the final 30 min of infection. Nuclear extracts were precipitated using the GAS oligo, resolved by SDS-PAGE, blotted with primary antibodies shown to the right and imaged with LI-COR specific secondary antibodies. Representative of three or more experiments with similar outcomes.

(B) ChIP of acetylated Histone H3 of HFF cells infected for 3 hr with type I (RH) wild type (red) and TgIST knockout (blue) ± IFN-γ (100 U/ml) for 2 hr. Data analyzed by qPCR with primers for GAS sites in promoter elements of genes shown. GAPDH served as control. Means ± S.D., n=6, *P < 0.05 and **P < 0.001. Student’s t-test.