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. 2016 Jul 16;47:71. doi: 10.1186/s13567-016-0355-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of orally fed BLS-mix on intestinal lymphocytes. A Representative dot plots showed the gating strategy for gut T-cell subpopulations. Flow cytometry analysis of the percentage of B CD4⁺CD8⁻, C CD4⁻CD8⁺, D CD4⁺CD8⁺, E CD4⁻CD8⁻ cells among intestinal CD3⁺ T cells. Peyer’s patch lymphocytes (PPLs), intraepithelial lymphocytes (IELs), and lamina propria lymphocytes (LPLs) were collected from jejunal and ileal tissues from the indicated pigs 1 week after F4⁺ ETEC/VTEC/EPEC challenge. Data are presented as the mean ± SEM for each tissue (n = 8 per group). *P < 0.05; **P < 0.01; ***P < 0.001.