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. 2016 Jul 16;47:71. doi: 10.1186/s13567-016-0355-8

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of orally fed BLS-mix on PKC-α, ZO-1, and occludin expression. Western blot analysis of PKC-α, zonula occludens-1 (ZO-1), and occludin expression in jejunal tissues collected from pigs 1 week after F4⁺ ETEC/VTEC/EPEC challenge. A Representative Western blot results for PKC-α in jejunal tissues collected from 1 pig of each group are shown. B Representative Western blot results for ZO-1 and occludin in jejunal tissues collected from 2 pigs of each group are shown. C Results are presented as the ratio of the intensity of the ZO-1 or occludin band to the intensity of the β-actin band. Data are expressed in arbitrary units (A.U.) as the mean ± SEM for each tissue (n = 8 per group). *P < 0.05; **P < 0.01.