Fig. 2.

Identification of sequence variations inc-kittranscript of canine HSAs. Following PCR amplification, sequences of amplicons were revealed by automated sequencing. a Distinct sequencing chromatograms of c-kit exon 9 were revealed between NCCand HSA samples. b The PCR products containing c-kit exon 9 were amplified from a NCCor a HSA and resolved in 3 % agarose gel. M, 100-bp DNA size markers. c Sequence alignment of two c-kit exon 9 variants. Dashed lines indicate deletion of nucleotides. Underlines indicate the deduced conserved di-nucleotide of splicing donors and acceptor sites