Table 2.
Sequences and locations of c-kit primers used in this study
| Designation | Sequence (5′-3′) | Primer location |
|---|---|---|
| 1F | CGATGAGAGGCGCTCGC | 27–34 |
| 1R | GGCGTAACACA TGAACACTCCAG | 891–913 |
| 2F | GCTGGCATCATGGTGACTTC | 819–838 |
| 2R | CATGGGTTTCTGTAGATACTTGTAGG | 1668–1693 |
| 3F | CACACCTTTGCTGATTGGCT | 1591–1610 |
| 3R | GATTCGACCATGAGTAAGGAGG | 2419–2440 |
| 4F | GGGTATGGCATTCCTGGC | 2359–2376 |
| 4R | GCTTCACACATCTTCGTGTACCA | 2937–2959 |
| AF | GCTCAGAGTCTATCGCAGCCACCG | 3–26 |
| AR | CTGCCTTCTCTGTGATCCATTCGTTG | 271–296 |
| BF | GCTGTCCAAGAAATTCACCCTG | 619–640 |
| BR | ATATTACTTTCATTGTCAGACTTGGG | 1124–1149 |
| CF | AAAACTCGTCTCTGTCACCGTCTG | 1422–1445 |
| CR | GATCTCCTCAACAACCTTCCACTG | 1691–1714 |
| DF | AAATCAGAGTTAATAGTCAGTGTCGG | 133–158 |
| DR | TTTATCCACATCGAGTCCACG | 729–749 |
| EX9F | CAACAATGTAGGCAGGAGTTCTG | 1495–1517 |
| EX9R | CAGCAAAGGTGTGAACAGGG | 1572–1591 |
F forward, R reverse
Annealing temperature dependent on combination of the primers: 55 °C for 1F and 1R, 56 °C for 2F and 2R, 52 °C for 3F and 3R, 61 °C for AF and AR, 54 °C for BF and BR, 57 °C for CF and CR, 54 °C for DF and DR, 57 °C for AF and BR, 55 °C for P2F and CR, 56 °C for CF and P3R, and 54 °C for EX9F and EX9R