Skip to main content
. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Cell Signal. 2016 Jun 11;28(9):1364–1379. doi: 10.1016/j.cellsig.2016.06.012

Figure 7. FL-VASP/239D negates the repressive effect of FL-VASP overexpression on ASM cell migration which is reversed with BAY60.

Figure 7

Figure 7

(A) Photomicrographs of naïve, and FL VASP mutants immediately following (time 0) and 16 hours (time 16hr) post-wounding scrape injury. (B) Quantification of naïve, and FL-VASP mutant ASM cell migration demonstrates FL-VASP/WT overexpression significantly reduces migration compared to naïve ASM cells 16 hours after injury. This inhibitory effect was abrogated with phospho-mimetic, FL-VASP/239D overexpression, but was restored with phospho-resistant, FL-VASP/ 239A overexpression. n=7 independent experiments performed in duplicate. * p < 0.05 vs. naïve controls. (C) BAY60 (10 µM) did not significantly impact FL-VASP/WT or FL-VASP/239A mutant ASM cell migration 16 hours post scrape injury when compared to each corresponding VEH-treated FL-VASP mutant; however, FL-VASP/239D overexpression in the presence of BAY60 significantly reduced migration compared to FL-VASP/239D alone. n=3 independent experiments in triplicate. * p < 0.05 vs. corresponding VASP mutant.