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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: Clin Cancer Res. 2016 Feb 4;22(14):3440–3450. doi: 10.1158/1078-0432.CCR-15-2710

Figure 3. The 161533 TriKE mediates NK cell survival and proliferation.

Figure 3

Post-HSCT recipient PBMCs were loaded with CellTrace proliferation dye and co-cultured with HL-60 Targets at a 5:1 (E:T) ratio for 7 days in the presence of 50 nM 1633 BiKE or 161533 TriKE. At the end of the incubation CD56+CD3− NK cells were assessed for viability through Live/Dead Near IR staining. A) An individual histogram and B) pooled analyses of viability in the NK cell population treated with the 1633 BiKE (gray) or 161533 TriKE (black) are shown. Individual dots represent separate post-HSCT samples treated with noted reagents (n=8). C) After 7 days, significantly fewer live cells remained in the 1633 BiKE condition precluding proliferation analysis. Proliferation in the 161533 TriKE condition was assessed by CellTrace dilution on live CD56+CD3− NK cells (black) and CD56−CD3+ T cells (gray). D) Pooled analyses depict the percent of dividing cells (top) and expansion index (bottom). The expansion index is calculated based on fold expansion within the population for a given amount of CellTrace dilution. Individual dots represent separate post-HSCT samples for noted populations (n=8).