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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Jun 15;1861(9 Pt A):1102–1110. doi: 10.1016/j.bbalip.2016.06.003

Fig. 2. Preparation of acyl CoA substrates.

Fig. 2

Step 1 shows the synthesis of mixed acyl anhydrides from the different carboxylic acids (R group-2-methylbutyrate and 2-ethylbutyrate). Step 2 shows the reaction of the mixed anhydride with an aqueous solution of CoA resulting in the formation of acyl CoA substrates according to the method outlined by Stadtman [23].