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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Jun 15;1861(9 Pt A):1102–1110. doi: 10.1016/j.bbalip.2016.06.003

Fig. 5. Concentration dependence of phosphate utilization by LmPtb.

Fig. 5

Ptb activity was determined at 25oC in the presence of varying phosphate concentrations (10 µM – 25 mM) and excess A) isobutyryl CoA (200 µM) (open circles) or B) isovaleryl CoA (200 µM) (open squares). Data were fitted to the Hill equation using Kaleidagraph software providing values for KM and kcat. Means of data from at least three experiments ± SEM were plotted as reaction rate versus phosphate concentration.