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ANA-reflex test procedure with patterns related to markers found in autoimmune liver diseases
| ANA-IIF pattern on HEp-2 cells | Reflex test(s) |
|---|---|
| Cytoplasmic reticular/AMA ≥1:160 | Anti-mitochondrial M2 or E3 or MIT3 |
| Multiple nuclear dots ≥1:160 | Anti-Sp100 |
| Nuclear envelope | Anti-gp210 |
| Cytoplasmic linear-actin ≥1:160 | IIF on kidney, stomach, and liver, to confirm anti-actin antibodies |
