Fig. 5.

a Schematic representation of the ELF3 gene. Positions of the selected mutations introducing premature stop codons on the A- and B-genome copies of the gene are indicated with red triangles. Exons are shown as gray rectangles and introns as black lines. b–e Heading time and spikelet number of tetraploid BC₂F₃ lines carrying four possible homozygous combinations of ELF3 and PPD1 alleles. Plants were grown either under LD (b, c) or SD conditions (d, e). ELF3 alleles: ELF3-WT (blue dashed lines), elf3-null (red lines). PPD1 alleles: PPD-A1a (allele with reduced photoperiodic response); PPD-A1b (photoperiod sensitive allele). Asterisks between lines indicate significant differences between ELF3 alleles; asterisks on top and below lines indicate significant differences between PPD1 alleles (*P < 0.05, **P < 0.01, ***P < 0.001, ns nonsignificant)