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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Mammary Gland Biol Neoplasia. 2016 May 19;21(1-2):9–17. doi: 10.1007/s10911-016-9354-7

Figure 2. Collection of interstitial fluid (IF) from tissues.

Figure 2

A IF was collected from different amounts of 4T1 breast tumor tissue. Correlation between the tumor tissue weight and the volume of IF collected is shown. B Removal of contaminating cells from IF. Cell numbers in IF were determined after centrifugation as indicated. C 10 μg of protein from lymph node tissue extracts (LN) or from IF were analyzed by western blotting with anti-actin antibody (lower panel) and stained with Ponceau S to visualize proteins (upper panel). Densitometry of the blot was assessed using Image J software, and the relative level of actin was normalized with equal protein amount of LN and IF.