Fig. 2.
Glutamic acid decarboxylase 67 (GAD67) and GAD65 mRNA quantified by qRT-PCR in cultured human bronchial airway epithelial (epith) and airway smooth muscle (ASM) cells demonstrates GAD67 is the predominant GAD isoform and is expressed in much greater abundance in airway epithelial cells. Note abundance of GAD67 mRNA in epithelium compared to ASM (reflected in lower ΔCt values). ΔCt was calculated as the (Ct of GAD67 or GAD65) −(the Ct of GAPDH) for each sample. n = 3–7, ***p < 0.001 when comparing GAD67 in human airway epithelium to GAD65 in epithelium or to GAD67 in human ASM