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. 2016 Jul 18;8:173. doi: 10.3389/fnagi.2016.00173

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Copyright © 2016 Scerbak, Vayndorf, Hernandez, McGill and Taylor.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lifespan-extending Alaskan berry and fungus treatments change endogenous ROS levels in young wild-type C. elegans. (A) The change in endogenous ROS measured by DCF-DA after treating young adults for 48 h with selected lifespan-extending Alaskan berry and fungus treatments is shown. The percent change on a logarithmic scale of hsp16.2::GFP expression in the pharynx region of young adults treated for 48 h with selected berry and fungus treatments relative to untreated control (B) and representative images (C–G) are also shown. Note the extreme increase in hsp16.2::GFP expression in response to heat shock. Bars represent mean ± standard error of the mean of each replicate, each with two or three technical replicates for the DCF-DA assay. Hashtag (p = 0.085) and asterisks (p < 0.06; one-way ANOVA with Tukey post hoc) denote significance from 0 μg/mL control.