Table II.
Methods of blood lipid determination
| Study name | Material | TC | HDL-C | TG | LDL-C |
|---|---|---|---|---|---|
| POL-MONICA Krakow | Plasma (frozen) | Manual, direct (Liebermann-Burchard procedure) | Manual, direct (Liebermann-Burchard procedure), after precipitation with MnCL2 and heparin | Manual, enzymatic | Calculated (Friedewald's formula) |
| POL-MONICA Warszawa | Plasma (frozen) | Manual, direct (Liebermann-Burchard procedure) | Manual, direct (Liebermann-Burchard procedure) after precipitation with MnCL2 and heparin | Manual, enzymatic | Calculated (Friedewald's formula) |
| WOBASZ | Serum (frozen) | Enzymatic-colorimetric test (CHOD/PAP) with cholesterol esterase, cholesterol oxidase, and 4-aminoantipyrine (Roche kit); INTEGA 400 automatic analyzer | Monochromatic colorimetric test with cholesterol esterase and cholesterol oxidase modified PEG after precipitation with MnCL2 and heparin (Roche kit); INTEGA 400 automatic analyzer | Enzymatic-colorimetric (GPO/PAD) with glycerol phosphate oxidase and 4-aminoantipyrine (Roche kit); INTEGA 400 automatic analyzer | Calculated (Friedewald's formula); if TG > 400 mg/dl monochromatic colorimetric test |
| Pilot HAPIEE | Plasma | Oxidation method with the MP3 Boehringer Mannheim chemical reagent kit in the Technicon RA-I000 automatic analyzer | Oxidation method with the MP3 Boehringer Mannheim chemical reagent kit in the Technicon RA-I000 automatic analyzer after precipitation with MnCL2 and heparin | Oxidation method with TG 30 Cormay chemical reagent kit | Calculated (Friedewald's formula) |
| HAPIEE | Plasma | Oxidation method with the MP3 Boehringer Mannheim chemical reagent kit in the Technicon RA-I000 automatic analyzer | Oxidation method with the MP3 Boehringer Mannheim chemical reagent kit in the Technicon RA-I000 automatic analyzer after precipitation with MnCL2 and heparin | Oxidation method with TG 30 Cormay chemical reagent kit | Calculated (Friedewald's formula) |
| NATPOL 2011 | Fasting serum (frozen) | Enzymatic/cholesterol esterase and cholesterol oxidases; Architect c8000 chemistry analyzer, Abbott Laboratories | Direct method – Accelerator Selective Detergent (ASD) with accelerated non-HDL-C oxidation and HDL-C dissolving; Architect c8000 chemistry analyzer, Abbott Laboratories | Enzymatic/glycerol kinase and glycerol phosphate oxidase; Architect c8000 chemistry analyzer, Abbott Laboratories | Calculated (Friedewald's formula) |