Figure 2.

NEDD8 is essential for adipose tissue differentiation of grafted preadipocytes. 3T3-L1 and 3T3-F442A preadipocytes, which stably expressed pLKO.1-sh-Control (Sh-Con) or pLKO.1-sh-NEDD8 (Sh-N8), were grafted into the subcutis of the abdomens of 9-week-old male BALB/c nude mice. Grafted fat pads were excised 5 weeks after grafting and then stained with hematoxylin and eosin (upper panel) or stained immunofluorescently with an anti-perilipin antibody (lower panel). Nuclei were stained with DAPI. Epididymal fat tissues were excised from the same mice and used as a positive control for well-differentiated fat tissues