Figure 8.

MLN4924 inhibits the expressions of PPARγ and lipid-storing genes in fat tissues. (a) MLN4924 downregulates PPARγ in fat tissues. Protein expression of PPARγ in epididymal fat tissues was analyzed by Western blotting (top panel). The band intensities (mean+S.D., n=3) were calculated using ImageJ and plotted in the bottom panel. (b) MLN4924 represses lipid-storing genes in fat tissues. RNAs prepared from epididymal fat tissues were subjected to RT-qPCR to check the expressions of Pparg, Fabp4, Cd36, Perilipin, Fasn, Acs, and Lpl mRNAs. The mRNA levels (mean+S.D., n=3) are shown as relative values to 18S RNA levels. *P<0.05