Table 2.
Toxicity and antitoxicity levels of CCC, CFCC, and CAF in D. melanogaster.
| CCC (mg/mL) | Survival (%) | CFCC (mg/mL) | Survival (%) | CAF (mM) | Survival (%) | |||
|---|---|---|---|---|---|---|---|---|
| Simple treatment(1) | Combined treatment(2) | Simple treatment | Combined treatment | Simple treatment | Combined treatment | |||
| 0 | 100 | 100 | 0 | 100 | 100 | 0 | 100 | 100 |
| H2O2 | — | 46.66 | H2O2 | — | 46.66 | H2O2 | — | 46.66 |
| 0.7 | 100 | 100∗(3) | 0.7 | 87.66 | 83.33∗ | 0.004 | 100 | 54Δ |
| 3 | 100 | 92∗ | 3 | 88.66 | 100∗ | 0.016 | 100 | 55.33Δ |
| 6 | 100 | 85.66∗Δ | 6 | 96.66 | 84.66∗ | 0.032 | 100 | 51Δ |
| 25 | 100 | 74.66∗Δ | 25 | 87.33 | 75∗Δ | 0.127 | 100 | 54.66Δ |
| 100 | 92 | 65∗Δ | 100 | 77∗(4) | 45.66Δ | 0.51 | 100 | 51.33Δ |
(1)Data are expressed as percentage of survival adults with respect to 300 untreated 72-hour-old larvae from three independent experiments. (2)Combined treatments using standard medium and 0.15 M hydrogen peroxide. (3)Asterisks (∗) indicate significant differences (one tail) with respect to the hydrogen peroxide control group and (4)untreated control group: ∗Chi-square value higher than 5.02 [35]. Delta letter (Δ) means significant differences between the same concentrations used in toxicity and antitoxicity assays comparing within the same treated substance.