Skip to main content
. 2016 Jun 23;115(2):203–211. doi: 10.1038/bjc.2016.190

Figure 1.

Figure 1

Y15 decreased viability and clonogenicity of lung cancer cell lines in a dose-dependent manner.(A) Expression of Y397-pFAK and FAK in lung cancer cell lines. Western blotting with Y397-pFAK and FAK was performed on different lung cancer cell lines. β-Actin was used as a loading control. Y397-pFAK and FAK levels are variable across different cell lines. (B) MTS assay. RAS-mutant (H1299, H727, H358, A549) or EGFR-mutant (H1650) cell lines were treated with Y15 or control FAK inhibitor C4 (not targeting Y397-pFAK) for 72 h. Y15 increased cell death in all cell lines, whereas control C4 did not. (C) Different lung cancer cells were treated with increasing doses of Y15 for 2 weeks and clonogenicity assay was performed. Y15 significantly decreased clonogenicity in a dose-dependent manner.