Figure 4.

ELISA analysis of EBV-LMP2 with specific IgG in immune sera and specific IgA in vaginal washing fluid. Mice (nine per group) were immunized with purified EBV-LMP2m recombinant protein at two-week intervals using the His-tag protein and PBS buffer as negative controls. Serum and vaginal secretions were collected for ELISA analysis at 0, 1, 3, 5, and 7 w. (a) The specific IgG response to EBV-LMP2m protein in the immune sera compared to the controls, F = 88.450, *P < .01 (5 W); F = 174.150, *P < 0.01 (7 W). (b) The specific IgG to EBV-LMP2 native protein in the immune sera compared to the controls, F = 12.495, **P < 0.05; F = 21.720, **P < 0.05. (c) The specific IgA to EBV-LMP2m protein in the vaginal mucosa compared to the controls, F = 60.300, **P < 0.05. (d) The specific IgA to EBV-LMP2 native protein in the vaginal mucosa compared to the controls, F = 37.226, *P < 0.01. The results are presented as the mean OD₄₅₀ absorbance value and standard deviation (error bars) for three experiments. The data were analyzed by one-way ANOVA.