Skip to main content
. 2015 Apr 27;13(4):514–523. doi: 10.1038/cmi.2015.33

Figure 1.

Figure 1

Generation and characterization of recombinant flagellin proteins. (a) Schematic representation of the recombinant WT and variant proteins used in this study. (b) IL-8 and MCP-1 were detected to confirm the bioactivity of the recombinant proteins on TLR5 activation. (c) Peritoneal macrophages were stimulated and analyzed using an IL-1β assay to confirm the effects of the recombinant proteins on NLRC4 activation. Data are presented as means ± SEM from triplicates of one experiment that was repeated at least three times.