Flagellin-specific antibody response activated via the NLRC4 or TLR5 pathway (a–d). C57BL/6 WT mice were immunized with 10 μg FliCΔ90-97:L3A, FliCΔ90-97, FliC-L3A, or FliC (six mice per group) on days 0 and 28. Sera were taken from each mouse on day 21, and FliCspecific IgG (a), IgG1 (b), IgG2a (c), and IgG2b (d) production was analyzed using ELISA. The results are presented as means ±6 SEM, and the data are representative of three independent experiments.