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. 2015 Jul 13;13(4):524–534. doi: 10.1038/cmi.2015.47

Figure 1.

Figure 1

Pdcd4 deficiency increased the expression of IL-10 in oxLDL-stimulated macrophages in vitro. Peritoneal macrophages from C57BL/6 and Pdcd4−/− mice were collected, cultured in vitro and stimulated with oxLDL (50 μg/mL) for the indicated time periods. (a) The expression of IL-10 mRNA was measured by real-time PCR. (b) The concentrations of IL-10 in the culture supernatants were detected by ELISA. (c–d) The mRNA levels and concentrations of IL-6 in the culture supernatants were measured by real-time PCR (c) and ELISA (d), respectively. (e–h) Peritoneal macrophages from Pdcd4+/+Apoe−/− and Pdcd4−/−Apoe−/− mice were stimulated with oxLDL (50 μg/mL) for the indicated time periods. The mRNA levels and concentrations of IL-10 (e, f) and IL-6 (g, h) in the supernatants were assessed by real-time PCR and ELISA, respectively. The data are shown as the means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. The data are derived from four mice per group.