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. 2015 Jul 13;13(4):524–534. doi: 10.1038/cmi.2015.47

Figure 3.

Figure 3

A high level of IL-10 expression was detected in the atherosclerotic lesions of the Pdcd4−/−Apoe−/− mice. Pdcd4+/+Apoe−/− and Pdcd4−/−Apoe−/− mice were fed a high fat diet from age 8 weeks to 16 weeks. The aortic arches with their atherosclerotic plaques were isolated. The expression of IL-10 was measured at the protein level by Western blot (a) and at the mRNA level by real-time PCR (b). The data are shown as the means ± SEM. *P < 0.05. (c) The aortic roots with their atherosclerotic plaques were isolated to prepare serial frozen sections. The cell nuclei were stained blue with Hoechst. IL-10 and CD68 were co-localized in the same sections by dual IF staining. CD68 (in green) was stained with rabbit anti-mouse antibody and FITC-conjugated anti-rabbit second antibody, and IL-10 (in red) was stained with rat anti-mouse antibody and TRITC-conjugated anti-rat second antibody. The merged image showed co-expression of IL-10 and CD68 (yellow) in the same cells. The cell indicated by the white arrows is shown magnified in the lower right corner. The data are derived from four Pdcd4+/+Apoe−/− mice and five Pdcd4−/−Apoe−/− mice.