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. 2015 Sep 21;13(4):535–550. doi: 10.1038/cmi.2015.77

Figure 7.

Figure 7

Inhibitors for the p38 MAPK, NF-κB, and ERK-MAPK pathways abolish NOD2/TLR2 ligand-induced activation of human basophils/eosinophils co-cultured with dermal fibroblasts. (a–d) Basophils co-cultured with dermal fibroblasts were pretreated with SB203580/BAY11-7082, followed by incubation with the NOD2/TLR2 ligands for an additional 20 h. The release of CXCL8 (a), CCL2 (b), and CCL5 (c) were determined. (d) ICAM-1 expression on dermal fibroblasts was analyzed and expressed as previously described. (e–h) Likewise, eosinophils co-cultured with dermal fibroblasts were pretreated with SB203580/BAY11-7082/U0126 and stimulated. The release of IL-6 (e), CXCL8 (f), CCL4 (g) were determined. (h) ICAM-1 expression on dermal fibroblasts was analyzed and expressed as described. SB, SB203580; BAY, BAY11-7082; U, U0126. The results are shown as the arithmetic mean plus SD. *P < 0.05, **P < 0.01 when compared between the denoted groups and the corresponding control groups with MDP, PGN, or LTA treatment.