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. 2016 Jul 18;7:277. doi: 10.3389/fimmu.2016.00277

Figure 2.

Figure 2

TAK1 is required for FcγRIIIb-induced NET formation. (A) human neutrophils were stimulated with 20 nM phorbol 12-myristate 13-acetate (PMA), or by cross-linking FcγRIIIb with mAb 3G8, and then incubated for 4 h. Some neutrophils were previously treated with 10 nM LL Z1640-2 (LLZ), a selective inhibitor of TAK1 (white symbols), or with only the solvent (DMSO) (black symbols). The relative amount of NETs was estimated from SYTOX® Green fluorescence in relative fluorescent units (RFU) every 5 min. Data are mean ± SEM of three experiments done in tetraplicates. (B) human neutrophils were previously treated with solvent alone (---) or with the 10 nM LL Z1640-2 (LLZ), before stimulating with nothing (medium), 20 nM PMA, or by cross-linking FcγRIIIb, and then incubated for 4 h. The relative amount of NETs was estimated from SYTOX® Green fluorescence in relative fluorescent units (RFU) at 4 h after stimulation. Data are mean ± SEM of three experiments done in tetraplicates. Asterisks denote conditions that are statistically different from control (p < 0.0003).