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. 2016 Jul 18;7:277. doi: 10.3389/fimmu.2016.00277

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Copyright © 2016 Alemán, Mora, Cortes-Vieyra, Uribe-Querol and Rosales.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MEK1 is required for FcγRIIIb-mediated ERK activation. Human neutrophils were left untreated (---), or were stimulated by cross-linking FcγRIIIb for 15 min, or by 20 nM phorbol 12-myristate 13-acetate (PMA). Some neutrophils were previously treated with 50 μM PD98059 (PD) or with 50 μM UO126 (UO), both selective inhibitors of MEK. Cell lysates were prepared after stimulation. Proteins were resolved by SDS-PAGE, and then Western blotted for (A,B) phosphorylated ERK (pERK) (upper panel) and for total ERK (lower panel); or for (C) phosphorylated-TAK1 (pTAK1) and for total ERK (lower panel). Data are representative of three separate experiments.