Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
HBMEC were treated with 25 μm 3b (left panels) in RPMI medium for 5 min at RT or were left untreated (right panels). After washing, the cells were exposed to 25 μm DIBO 488 alkyne and click reaction was performed for 5 min at RT (upper panels) or for 60 min at 37 °C (lower panels). Cells were washed three times with PBS and kept at RT until microscopic analysis.
