Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 18;7:96. doi: 10.3389/fendo.2016.00096

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Maben, Malik, Jiang and Hinkle.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Importance of MRAP Lys residues for function, orientation, and stability. CHO cells were transfected with no accessory protein, wild-type MRAP or (4K to R)MRAP with the four native Lys residues mutated to Arg. (A) Surface expression of the N-terminal V5 and C-terminal Flag epitopes of MRAP and (4K to R)MRAP, and surface expression of co-transfected HA-tagged MC2 receptor. Results are normalized to values with wild-type MRAP. (B) cAMP responses to ACTH in cells co-transfected with MC2 receptor and CRE-luciferase. (C) Cells expressing MRAP or (4K to R)MRAP were incubated for the times shown with 10 μg/ ml cycloheximide (CHX), 50 μM MG132 or both. Lysates were run on gels and blotted for Flag epitope. *P < 0.05 vs. corresponding value in cells expressing wild-type MRAP.