(a–g) Normalized mean pre- (black circles) and post-bleach (grey circles) spectra of pigments measured using MSP from emu retinal photoreceptor outer segments. Pre-bleach spectra are overlayed with best-fit rhodopsin (vitamin A1) templates (black line). Post-bleach spectra are fitted with variable-point running average (grey line). (h) Histogram shows the spectral distribution of the wavelength of maximum absorbance (λmax) values for individual photoreceptor outer segments that were used to generate the mean spectra. The data for the distribution of LWS pigment λmax values includes measurements from LWS single cones, as well as both the principal and accessory members of double cones. λmax values are UVS 367 nm, SWS 453 nm, MWS 502 nm, and LWS 562 nm. UVS, ultraviolet-sensitive; SWS, short-wavelength-sensitive; MWS, medium-wavelength-sensitive; LWS long-wavelength-sensitive.