Figure 3. Maximum likelihood (ML) tree of the fatty acyl reductase proteins from Lepidoptera.

The ML analysis was computed using MEGA (v.6.0)⁴⁹. [The Jones-Taylor-Thornton (JTT) model for the ML heuristic search methods was the Nearest-Neighbor Interchange]. The Lepidopteran FAR-like sequences were retrieved from the GenBank, EST and TSA databases using BLASTx searches, and SexpgFAR I and SexpgFAR II were used as the query. The protein sequences were aligned using MUSCLE⁴⁸. The branch containing Jojoba FAR²⁸ was used as outgroup to root the tree. Other FAR-like proteins characterized from S. exigua are shown in blue. The pgFAR clade is highlighted in yellow. The GenBank accession numbers are indicated.