Figure 4. Analysis of the yeast construct functional assay showing the fatty alcohol production of SexpgFAR I and SexpgFAR II from S. exigua and SlitpgFAR I and SlitpgFAR II from S. littoralis.
The recombinant yeast strain S. cerevisiae was supplemented with 0.5 mM concentrations of various fatty-acyl methyl ester (FAME) substrates for 48 h at 30 °C, and the n-hexane extracts of the yeast pellets were subjected to GC-MS analysis. The fatty alcohol production was calculated using a previously described method11. The bars represent the SEMs of fatty alcohol production (in nanograms) of 3 biological replicates. The bars with different letters within each series are significantly different (P < 0.05).