Table 2. Summary of the pgFAR activities toward different substrates.
| Substrate tested | SexpgFAR I | SlitpgFAR I | SexpgFAR II | SlitpgFAR II |
|---|---|---|---|---|
| Saturated fatty acids | ||||
| C14:Acyl/C16:Acyl | C16:Acyl | C16:Acyl | C14:Acyl | C14:Acyl |
| C14:Acyl | C14:Acyl | C16:Acyl | C16:Acyl | |
| Monounsaturated fatty acids | ||||
| Z11-16:Acyl | Z11-16:Acyl | Z11-16:Acyl | Z12-14:Acyl | Z9-14: Acyl |
| E11-14:Acyl | E14-16:Acyl | E14-16:Acyl | Z9-14: Acyl | Z12-14:Acyl |
| Z9-14:Acyl | E11-14: Acyl | E11-14: Acyl | E11-14: Acyl | E11-14:Acyl |
| E12-14:Acyl | Z11-14: Acyl | Z11-14: Acyl | E12-14:Acyl | Z11-14:Acyl |
| Z12-14:Acyl | Z11-16:Acyl | E12-14:Acyl | ||
| Z7-12:Acyl* | Z11-14: Acyl | Z11-16:Acyl | ||
| E14-16:Acyl | ||||
| Z5-10:Acyl* | ||||
| Z11-14:Acyl | ||||
| Diunsaturated fatty acids | ||||
| Z9Z11-14:Acyl | — | — | Z9E12-14:Acyl | Z9Z12-14:Acyl |
| Z9E12-14:Acyl | Z9Z12-14:Acyl | Z9E12-14:Acyl | ||
| Z9E11-14:Acyl | Z9E11-14:Acyl | Z9E11-14:Acyl | ||
| E10E12-14:Acyl | E10E12-14:Acyl | E10E12-14:Acyl | ||
| Z9Z12-14:Acyl | Z9Z11-14:Acyl | Z9Z11-14:Acyl | ||
The total fatty alcohol production from three biological replicates was quantified11, and based on the quantity of the fatty alcohols produced in yeast assays, the preferred substrates were arranged from highest to lowest in each row.
Not reducing to alcohol; —indicate no enzyme activity.