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. 2016 Jul 18;6:29903. doi: 10.1038/srep29903

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(a) RT-PCR was performed to confirm UL23 gene transcription. The analysis of GAPDH served as cellular control. The PCR was conducted in presence (+) and absence (−) of reverse transcriptase to exclude DNA contamination. (b) Immunoblotting was performed to confirm TK protein expression. The analysis of gD protein served as viral-load control and beta-actin as cellular control. (c) Immunofluorescence microscopy revealed TK protein expression in infected Vero cells. Plaques by EGFP-tagged virus strains (first column) were stained with anti-TK antibody (second column). The third column displays the merged images.