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. 2016 Jul 18;6:29903. doi: 10.1038/srep29903

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Copyright © 2016, Macmillan Publishers Limited

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A constant number of 2 × 10⁵ Vero cells were infected with a MOI of 0.01 and 3.3% of the eluate volume from whole cell DNA extracts obtained one day p.i. was used as template for qPCR. (a) The HSV1(17⁺)Lox strain was tested with ACV concentrations ranging from 35.2 to 0 μM. HSV1(17⁺)Lox was graded as susceptible reference strain. (b) The TK-modified strains were tested with a high concentration (35.2 μM, filled bars) or in absence (0 μM, empty bars) of ACV. Data are plotted as the mean and SD of three measurements from one representative experiment.