Figure 2.
(A) Exemplary LC ESI MS TIC chromatogram of a single gel slice peptide extract corresponding to a control retina lysate highlighting the exemplary identification of ANT3 at RT 31.01 min. within the 50 min gradient (B). MS/MS fragment spectrum of 617.34 m/z (2+; MH+ 1233.69 Da) contributing to the identification of ANT3 (C). Elution range of the unique ANT3 reporter peak at 617.34 m/z between RT 30–80 min corresponding to an in-solution digest of a control retina lysate pool (D). Exemplary MS spectra at three RTs showing the ANT3 reporter peak 617.34 m/z corresponding to the amino acid sequence: DFLAGGIAAAISK.