Table 2. Subset of representative human retinal candidate proteins selected for AIMS validation in retina sample pools.
| Protein | Gene names | Mass [Da] | m/z | Amino acid sequence | Charges | Glaucoma/Control | Valid |
|---|---|---|---|---|---|---|---|
| ADP/ATP translocase 3 (ANT3) | SLC25A6 | 1232,68 | 617,34 | DFLAGGIAAAISK | 2 | 0,38 | √ |
| PC4 and SFRS1-interacting protein (DFS70) | PSIP1 | 1631,76 | 816,88 | GFNEGLWEIDNNPK | 2 | 0,80 | √ |
| Methyl-CpG-binding protein 2 (MeCp2) | MECP2 | 1954,89 | 978,45 | VGDTSLDPNDFDFTVTGR | 2 | 0,65 | √ |
| α-crystallin B chain (HspB5) | CRYAB | 1164,65 | 583,33 | VLGDVIEVHGK | 2 | 1,08 | × |
| Arrestin-C (cArr) | ARR3 | 1821,94 | 911,97 | VQFAPPEAGPGPSAQTIR | 2 | 0,99 | × |
Analysis of technical replicate runs (N = 4; N = 4 individuals/pool sample) supported glaucoma related significant level diminishment of ANT3, MeCp2 and distinct decline of DFS70 highlighted by check mark in the column.