Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 18;6:29884. doi: 10.1038/srep29884

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) IFN-γ level determination in the control and DM of CD-1 and 129Sv mice strains, n = 5 were analyzed in each data set. (B) Gene expression of IFN-γ mRNA expression level in kidney of the control and DM of the CD-1 and 129Sv strains, n = 4 were analyzed in each data set. (C) IFN-γ level in the plasma of control, DM and AcSDKP-treated diabetic mice, n = 5 were analyzed in each data set. (D) qPCR analysis of IFN-γ mRNA gene expression in the kidneys of the control, DM and AcSDKP-treated diabetic kidney, n = 5 were analyzed in each data set. (E) IFN-γ level in the plasma of control, DM and linagliptin treated diabetic mice, n = 5 were analyzed in each data set. (F) qPCR analysis of IFN-γ mRNA gene expression in the kidneys of the control, DM and linagliptin-treated diabetic kidneys, n = 6 were analyzed in each data set. (G) qPCR gene expression analysis of IFN-γ mRNA level in the HMVECs transfected with antagomirs of miR-29s, n = 6 were analyzed in each data set. (H) Western blot analysis of the mimic treatment of miR-29s in the HMVECs. Representatives from 5 blots are shown here. (I) Western blot analysis of the antagomir treatment of miR-29s in the HMVECs, with or without neutralizing antibody for IFN-γ antibody and quantification of densitometric analysis. Representative from 5 blots are shown here. (J–L) qPCR analysis of miR-let-7s after transfection of the antagomir treatment of miR-29s in the HMVECs, with or without neutralizing IFN-γ antibody, n = 6 were analyzed in each data set. Hs_RNU6 was used as internal control. The data are expressed as the means ± s.e.m and are included in the graph.