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. 2016 Jul 18;6:29969. doi: 10.1038/srep29969

Figure 6. Confirmation of DHMD’s antiviral effect against HCV particle attachment using ELISA-based virus binding analysis.

Figure 6

(a) Schematics of the ELISA-based virus binding assay. (b) Huh-7.5 cells were treated with DHMD (50 μM) and HCVcc (MOI = 0.01) at 4 °C for 3 h, then washed with PBS before fixation and analysis for cell surface-bound virus particles by ELISA using anti-HCV E2 antibody. Dashed line indicates baseline signals. DMSO = 0.5%; anti-CD81 = 10 μg/ml; S29 cell = CD81-deficient Huh-7 derivative. Data shown are means ± SEM (*P < 0.05) from 3 independent experiments.