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. 2016 Jul 16;22:816–826.

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Copyright © 2016 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Immunohistochemical analysis of GFAP. A: Representative slice showing glial fibrillary acidic protein (GFAP) expression in healthy (n = 4) and ischemia/reperfusion (I/R)-injured retinas. Scale bar, 50 μm. B: Intensity index (GFAP) calculations revealed significantly lower GFAP levels in I/R-injured retinas following mutant (triple Y-F) self-complementary adenovirus-associated virus type 2 vector encoding brain-derived neurotrophic factor (tm-scAAV2- BDNF) treatment (n = 4) than in the no vehicle (n = 3; **p<0.01) retinas or the retinas treated with tm-scAAV2-GFP (n = 3; **p<0.01). Bars depict means ± standard deviation (SD).