Figure 1. MAP1B is expressed in mature WT neurons and in adult brain.

(A) Immunocytochemistry of a mature WT neuron stained against MAP1B and the presynaptic marker Synaptophysin I. Red arrows indicate dendrites innervated by presynaptic terminals, and red arrowheads indicate axons. Scale bar 10 μm. (B) MAP1B co-distributes with the somato-dendritic marker MAP2 (upper panel), and with the axonal marker tau-1 (lower panel). Scale bars 20 μm. (C) Immunoblot of mice adult brain tissue using antibodies against total MAP1B (upper panel) and MAP1B light chain (MAP1B-LC1, middle panel). 30 μg of proteins were loaded in each lane, and tubulin expression was used as a loading control. OB: olfactory bulb; Crb: cerebellum; Cx: cortex; Hipp: hippocampus. (D) MAP1B is present in both the presynaptic (Synaptophysin positive) and postsynaptic (PSD-95 positive) synaptosomal fractions derived from WT mice adult brain. For (C,D) gels for each antibody were run under same experimental conditions. Western blot raw data is presented in Supplementary information.