Table 1. The Hsf genes identified from the P. euphratica.
| Gene Name | ID | Map position (bp) | Length (aa) | MW (kDa) | pI | n.c.r. (%) | p.c.r. (%) | I.I. | Stability | A.I. | GRAVY |
|---|---|---|---|---|---|---|---|---|---|---|---|
| PeuHsf-A1a | CCG006597.1 | scaffold1541.1:1132–3828(+) | 491 | 53.7 | 4.95 | 63 (12.8%) | 41 (8.4%) | 61.54 | unstable | 72.75 | −0.553 |
| PeuHsf-A1b | CCG033924.1 | scaffold96.1:588160–589431(−) | 423 | 46.6 | 5.53 | 50 (11.8%) | 38 (9%) | 65.03 | unstable | 65.93 | −0.626 |
| PeuHsf-A1c | CCG014424.1 | scaffold25.1:1905477–1910713(−) | 510 | 56 | 4.79 | 67 (13.1%) | 45 (8.8%) | 64.06 | unstable | 67.73 | −0.647 |
| PeuHsf-A1d | CCG024768.1 | scaffold49.1:1143998–1146694(−) | 523 | 57.2 | 4.95 | 64 (12.2%) | 41 (7.8%) | 62.77 | unstable | 76.33 | −0.486 |
| PeuHsf-A2 | CCG009416.1 | scaffold19.1:1223913–1226045(−) | 425 | 48.1 | 5.04 | 65 (15.3%) | 47 (11.1%) | 53.2 | unstable | 71.29 | −0.573 |
| PeuHsf-A3 | CCG023633.1 | scaffold4512.1:713–3120(+) | 522 | 58.2 | 4.78 | 82 (15.7%) | 51 (9.8%) | 66.9 | unstable | 65.17 | −0.613 |
| PeuHsf-A4a | CCG026694.1 | scaffold56.1:184555–187033(+) | 404 | 45.9 | 5.22 | 57 (14.1%) | 43 (10.6%) | 56.17 | unstable | 65.17 | −0.801 |
| PeuHsf-A4b | CCG015090.1 | scaffold26.1:1129045–1132041(−) | 444 | 51 | 5.62 | 64 (14.4%) | 49 (11%) | 62.36 | unstable | 65.43 | −0.794 |
| PeuHsf-A4c | CCG030319.1 | scaffold739.1:61353–63562(−) | 427 | — | — | 61 (14.3%) | 45 (10.5%) | 55.87 | unstable | 63 | −0.772 |
| PeuHsf-A5a | CCG001724.1 | scaffold1039.1:58159–61226(−) | 485 | 54.1 | 5.96 | 63 (13%) | 56 (11.5%) | 56.93 | unstable | 67.2 | −0.783 |
| PeuHsf-A5b | CCG019200.1 | scaffold323.1:258192–261325(−) | 489 | 54.6 | 5.92 | 65 (13.3%) | 57 (11.7%) | 59.16 | unstable | 70.43 | −0.785 |
| PeuHsf-A5c | CCG019201.1 | scaffold323.1:281255–285809(−) | 737 | 81.2 | 7.02 | 82 (11.1%) | 81 (11%) | 54.67 | unstable | 77.84 | −0.577 |
| PeuHsf-A6a | CCG007863.1 | scaffold17.1:1250083–1251797(−) | 358 | 41.4 | 5.14 | 58 (16.2%) | 43 (12%) | 54.42 | unstable | 67.23 | −0.788 |
| PeuHsf-A6b | CCG000940.1 | scaffold1.1:8456623–8458194(+) | 331 | 38.2 | 5.08 | 56 (15.8%) | 41 (11.5%) | 59.91 | unstable | 72.15 | −0.736 |
| PeuHsf-A7a | CCG013195.1 | scaffold23.1:595273–598538(−) | 355 | 40.2 | 5.33 | 55 (15.5%) | 45 (12.7%) | 66.56 | unstable | 64.31 | −0.882 |
| PeuHsf-A7b | CCG017804.1 | scaffold30.1:760950–762628(−) | 359 | 41.2 | 5.44 | 54 (15%) | 47 (13.1%) | 55.04 | unstable | 68.75 | −0.788 |
| PeuHsf-A7c | CCG034159.1 | scaffold988.1:37988–39666(−) | 359 | 41.2 | 5.44 | 54 (15%) | 47 (13.1%) | 55.25 | unstable | 68.75 | −0.782 |
| PeuHsf-A8 | CCG033049.1 | scaffold9.1:2064027–2067752(−) | 392 | 44.7 | 4.72 | 66 (16.8%) | 37 (9.4%) | 43.46 | unstable | 72.6 | −0.698 |
| PeuHsf-A9 | CCG013896.1 | scaffold240.1:34097–35820(+) | 501 | 55.8 | 5.28 | 76 (15.2%) | 58 (11.6%) | 57.98 | unstable | 71.86 | −0.586 |
| PeuHsf-B1 | CCG009216.1 | scaffold188.1:576141–580111(+) | 288 | 31.4 | 4.73 | 51 (17.7%) | 35 (12.2%) | 38.99 | unstable | 58.26 | −0.903 |
| PeuHsf-B2a | CCG001341.1 | scaffold1000.1:34084–35186(+) | 294 | 32.4 | 5.02 | 44 (15%) | 34 (11.6%) | 49.29 | unstable | 68.54 | −0.669 |
| PeuHsf-B2b | CCG024466.1 | scaffold48.1:934135–936766(−) | 340 | 36.6 | 4.95 | 47 (13.8%) | 35 (10.3%) | 53.42 | unstable | 69.76 | −0.552 |
| PeuHsf-B2c | CCG005948.1 | scaffold148.1:57661–59281(+) | 287 | 31.9 | 5.02 | 47 (16.4%) | 37 (12.9%) | 52.39 | unstable | 79.06 | −0.507 |
| PeuHsf-B2d | CCG007214.1 | scaffold16.1:1230405–1231507(+) | 294 | 32.4 | 5.02 | 44 (15%) | 34 (11.6%) | 49.29 | unstable | 68.54 | −0.669 |
| PeuHsf-B3 | CCG001458.1 | scaffold101.1:1374018–1376473(−) | 228 | 26.5 | 7.62 | 37 (16.2%) | 38 (16.7%) | 55.59 | unstable | 72.28 | −0.736 |
| PeuHs -B4a | CCG027759.1 | scaffold6.1:2805693–2807677(+) | 364 | 40.4 | 8.15 | 32 (8.8%) | 34 (9.3%) | 51.71 | unstable | 73.9 | −0.466 |
| PeuHsf-B4b | CCG020996.1 | scaffold38.1:136369–138015(+) | 271 | 31.5 | 6.49 | 34 (12.5%) | 31 (11.4%) | 56.94 | unstable | 71.51 | −0.585 |
| PeuHsf-B4c | CCG010253.1 | scaffold2.1:1006270–1007951(+) | 368 | 41 | 7.72 | 33 (9%) | 34 (9.2%) | 55.68 | unstable | 69.16 | −0.552 |
| PeuHsf-B4d | CCG023235.1 | scaffold44.1:1597707–1599573(−) | 270 | 31.3 | 7.18 | 32 (11.9%) | 32 (11.9%) | 58.82 | unstable | 68.93 | −0.651 |
| PeuHsf-B5a | CCG006244.1 | scaffold15.1:1276216–1278351(+) | 209 | 24.1 | 9.24 | 26 (12.4%) | 33 (15.8%) | 55.35 | unstable | 71 | −0.649 |
| PeuHsf-B5b | CCG032077.1 | scaffold84.1:65593–67546(−) | 207 | 23.9 | 9.28 | 22 (10.6%) | 30 (14.5%) | 52.67 | unstable | 57.58 | −0.744 |
| PeuHsf-C1 | CCG004861.1 | scaffold135.1:519792–520919(+) | 338 | 37.8 | 5.92 | 40 (11.8%) | 37 (10.9%) | 49.6 | unstable | 75.86 | −0.415 |
Notes: pI, isoelectric point; n.c.r., total number of negatively charged residues (Asp + Glu); p.c.r., total number of positively charged residues (Arg + Lys); I.I., instability index; A.I., aliphatic index; GRAVY, grand average of hydropathicity.