Figure 3.

Endothelial cells in brain vasculature undergo apoptosis in experimental lupus. MRL ^+/+ mice and MRL/lpr mice were killed at 18 weeks of age. Brains were harvested, cryosectioned, immunostained and observed using a Zeiss confocal microscope. Representative sections were stained with Alexa 594 labelled anti‐caspase‐3 and observed at × 60 under oil. Lining of the vasculature was stained using Alexa 488‐labelled agglutinin1. Overlay (c) shows increased caspase 3 activity in the brain vasculature of MRL/lpr mouse brain (b) compared with controls (a).