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. 2016 Jun 29;148(4):326–338. doi: 10.1111/imm.12611

Figure 4.

Figure 4

T helper type 1 (Th1) and Th17 differentiation in mice whose microRNA (miR) 223 was deficient globally is impaired following experimental autoimmune encephalomyelitis (EAE) induction. (a) At day 22‐post MOG‐immunization, animals were killed and spinal cords were isolated. RNAs isolated from spinal cords were subjected to quantitative PCR analysis of Th1 cytokine interferon‐γ (IFN‐γ) and Th17 cytokine interleukin‐17a (IL17a) mRNA levels (n = 5). (b) Wild‐type and miR223−/− mice (n = 4) at an age similar to the mice used in (a) but not immunized with MOG were killed and spinal cords were collected for RNA isolation. The mRNA levels of IFN‐γ and IL‐17a mRNA levels were determined by quantitative PCR. (c–e) Spinal cords sections were subjected to immunostaining with secondary antibody only (negative control) (c) or IL‐17a antibody (d) or IFN‐γ antibody (e). *P < 0·05, ***P < 0·001.