Table 2.
List of primers used for polymerase chain reaction and real time PCR analysis
| Genes | Amplified sequences | Primer sequence (5'→3') | Annealing temperature (°C) | Product length (bp) | |
|---|---|---|---|---|---|
| Conventional PCR | β-ACTIN | - | F: CGTGACATTAAGGAGAAGCTGTGC | 55 | 375 |
| R: CTCAGGAGGAGCAATGATCTTGAT | |||||
| DPY19L2 | Exon 1 | F: GGCCAACTTCTTTCTACTCGGAC | 65 | 504 | |
| R: GACCCAGCTCCACCATACTCCTT | |||||
| Exon 4 | F: CAAAATAGCGAGAAGTGATTAG | 54 | 414 | ||
| R: TTCTACTCAACTATAAGGATACAC | |||||
| Exon 5 | F: AGCTTCATCCATGTCACTAT | 60 | 432 | ||
| R: AGCCTTCTCAGAAAACTATTTT | |||||
| Exon 6 | F: GGGTAAATAATTAAACACAGCA | 57 | 462 | ||
| R: AAACAACAGAATAAAAGGGAT | |||||
| Exon 7 | F: AATTTATACGTACACTTTTTAGAATTA | 55 | 420 | ||
| R: ATTTAAACATTTCAATCAACATGC | |||||
| Exon 8 | F: TGGACATGGTAGTTAATTGCTG | 55 | 371 | ||
| R: TCCCAAAGTGCTGAATTGAA | |||||
| Exon 11 | F: AACCTCCTCAAGTGACTTAG | 53 | 516 | ||
| R: TTGGCCAAGAGTCATT | |||||
| Exon 22 | F: GTGTCTGTTATTAAAGCTTGTG | 59 | 313 | ||
| R: ATTGTCTCTAGACAGCAATACAT | |||||
| Break point “a” | - | F: ATGCCATGTTGCCTGCT | 62 | 1700 | |
| R: TCTTCTGGGAAAGGTATTATCGTAG | |||||
| SPATA16 | Exon 4 | F: AATTCTTTGCCATTGTCATATC | 58 | 635 | |
| R: GGTCAAGCGCATTTCTATTAC | |||||
| PICK1 | Exon 13 | F: TGGGCTGCCATCCATGATC | 66 | 568 | |
| R: GCTCCCAGGCTCCGTCCTC | |||||
| PROTAMINE1 | - | F: CCCCTGGCATCTATAACAGGCCGC | 60 | 530 | |
| R: TCAAGAACAAGGAGAGAAGAGTGG | |||||
| Real-time PCR | β-ACTIN | - | F: AGATGCGTTGTTACAGGAAG | 60 | 92 |
| R: TGTGTGGACTTGGGAGAG | |||||
| DPY19L2 | - | F: GACCCAGCTCCACCATACTCCTT | 60 | 144 | |
| R: TTCCATCTCCTCCTCTACCTCCG | |||||