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DPY19L2 genotype achieved by quantitative real-time PCR for the family members of Globo 21 and Globo 22
| Subject | Quantities ratio | 2-∆∆Ct ratio |
|---|---|---|
| Father (P1) | 0.522924 | 0.4 |
| Mother (P2) | 0.408959 | 0.4 |
| Brother-1 (B1)* | 1.006982 | 1 |
| B1 sibling | 0.957183 | 0.9 |
| Brother-2 (B2)* | 0.499332 | 0.4 |
| B2 wife | 0.414017 | 0.4 |
| Globo22 wife | 1.037303 | 1 |
| Globo21 wife | 0.488268 | 0.4 |
*; B1 and B2 are 2 fertile patient’s (Globo21) brothers, Ct; Threshold cycle, and PCR; Polymerase chain reaction.
