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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Cancer Immunol Res. 2016 May 16;4(7):631–638. doi: 10.1158/2326-6066.CIR-15-0221

Table 1.

Hu3F8-IgG1: Affinities for FcRs*

ka1 (1/Ms) kd1 (1/s) ka2 (1/s) kd2 (1/s) KD (M)
CD16A(158V) 1.34E+05 2.12E-01 1.05E-02 8.97E-03 7.26E-07*
CD16A(158F) 2.09E-06**
CD16B 2.03E-06**
CD32A(131R) 7.86E-07**
CD32A(131H) 5.41E-07**
CD32B 2.40E-06**
*

The results were fitted either with Two-State Reaction: A+B=AB=AB*, KD = (kd1/ka1)/(1 + ka2/kd2),

**

or with steady-state equilibrium analysis if the affinity was too low (KD ≥ 1 uM) when determined by Two-State Reaction method. For CD32A(131H), where the KDs were near identical or identical when analyzed by either steady-state equilibrium or Two-State Reaction analysis, only the steady-state equilibrium result was shown for comparability with that of CD32A(131R).