Table 1.
ka1 (1/Ms) | kd1 (1/s) | ka2 (1/s) | kd2 (1/s) | KD (M) | |
---|---|---|---|---|---|
CD16A(158V) | 1.34E+05 | 2.12E-01 | 1.05E-02 | 8.97E-03 | 7.26E-07* |
CD16A(158F) | – | – | – | – | 2.09E-06** |
CD16B | – | – | – | – | 2.03E-06** |
CD32A(131R) | – | – | – | – | 7.86E-07** |
CD32A(131H) | – | – | – | – | 5.41E-07** |
CD32B | – | – | – | – | 2.40E-06** |
The results were fitted either with Two-State Reaction: A+B=AB=AB*, KD = (kd1/ka1)/(1 + ka2/kd2),
or with steady-state equilibrium analysis if the affinity was too low (KD ≥ 1 uM) when determined by Two-State Reaction method. For CD32A(131H), where the KDs were near identical or identical when analyzed by either steady-state equilibrium or Two-State Reaction analysis, only the steady-state equilibrium result was shown for comparability with that of CD32A(131R).