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Scaffold preparation. (A) Schematic of nCS-fibrin plug preparation, MSC loading, and VEGF/FGF9 conjugating. nC, nanocalcium sulfate; Fb, fibrin; F, FGF9; V, VEGF; M, MSCs. (B) Representative images of nCS disks before and after fibrin encapsulation, and subcutaneous implantation. (C) Scanning electron microscopy view of nanocalcium sulfate (nCS), fibrin (Fb), MSC seeding on fibrin, and nanocalcium sulfate/fibrin mixture (nC/Fb). Color images available online at www.liebertpub.com/tea
