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. 2016 Jul 1;19(7):701–709. doi: 10.1089/jmf.2016.0010

FIG. 2.

FIG. 2.

RT-PCR validation of ECM-related genes derived from microarray analysis following oral PVS supplementation. Expression levels of selected collagen genes identified using GeneChip® analyses were independently verified using real-time quantitative (Q) PCR. The effects of oral PVS supplementation (250 mg/b.i.d.) were measured during the course of all visits; V1, baseline; V2, after 8 weeks of oral supplementation; V3a, additional (following 8 weeks of initial supplementation) 4 weeks of oral supplementation and exercise, sample collection before the final stint of exercise; and V3b, same as study visit 3, sample collected 30 min post-final bout of exercise. Data are mean ± SEM (n = 16); *P < .05 compared with the baseline visit and †P < .05 compared with 8 weeks. No significant changes were observed between pre and post 30-min final exercise on week 12. PCR, polymerase chain reaction.