Table 1.
Immunohistochemistry Markers Used to Identify Cellular Populations and Senescence
| Protein marker | Target/function | Primary antibody | Catalog number | Dilution factor |
|---|---|---|---|---|
| S100β | Schwann cells | Polyclonal Rabbit Anti-S100β (Dako) | Z0311 | 1:500 |
| CD90 | Fibroblasts, endothelial cells, T cells, and NK cells | Monoclonal Mouse Anti-CD90/Thy1 (Abcam) | AB225 | 1:1000 |
| Fibronectin | Endoneurial cells (stromal cells) | Polyclonal Goat Anti-Fibronectin (Santa Cruz Biotechnology) | SC-6952 | 1:100 |
| CD68 | Macrophages | Monoclonal Mouse Anti-CD68 [ED1] (Abcam) | AB31630 | 1:100 |
| p16 | Cell cycle regulator | Monoclonal Mouse Anti-CDKN2A/p16INK4a (Abcam) | AB54210 | 1:250 |
| p53 | Cell cycle regulator | Monoclonal Mouse Anti-p53 (Abcam) | AB26 | 1:250 |
| Ki67 | Cellular proliferation marker | Polyclonal Rabbit Anti-Ki67 (EMD Millipore) | AB9260 | 1:300 |